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Gel electrophoresis

Loading and Running an SDS-PAGE Gel

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Learn about the steps involved in loading and running an SDS-PAGE gel. Practise loading protein samples, adjusting power pack settings and processing the gel by choosing how best to trim it using a wedge tool.

Learning outcomes

  • Familiarise yourself with the appearance of an SDS gel tank
  • Load a protein sample into an SDS-PAGE gel
  • Set up equipment for running an SDS-PAGE gel
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Skills

Using Instruments and Equipment

  • What are the main features of the set-up once the gel is placed in an electrophoresis tank 
  • How to position the pipette tip to carefully deposit the sample in the well of the gel 
  • What are the typical components of the sample loading buffer 
  • Why it is important to correctly wire the electrodes to the tank
  • Where the gel should be trimmed after it has finished running

Obtaining and Recording Data

  • Why a protein ladder is loading alongside the samples 
  • How the voltage selected can affect the running of the gel and the quality of the results

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Dr. Sharon Williams
Coventry University

“It has been a great experience to implement the online material and see the impact. The students found it very helpful and engaging. It takes away the fear of uncertainty before coming to the lab.”

Details

Degrees

Biochemistry
Biological science
Biomedical science
Forensic science
Pharmacological science

Modules

Biological chemistry

Topic

Gel electrophoresis

Purchase option

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