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Gel Electrophoresis

SDS-PAGE Staining

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Learn about the steps involved in visualising protein bands on an SDS-PAGE gel, including key parts of the staining and destaining process. Examine and interpret the resulting bands to correctly label the molecular weight and describe the bands in the sample lane.

Learning Outcomes

  • Stain and then destain the SDS-PAGE gel in order to visualise proteins
  • Examine the resulting bands and test your understanding by answering some questions
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Obtaining and Recording Data

  • Why an SDS-PAGE gel is stained 
  • Why the gel is washed with destaining solution
  • What is meant by molecular weight standards 

Interpreting and Analysing Data

  • How the protein ladder bands of different molecular weights migrate in relation to each other
  • How to identify the number of different protein species in a sample 
  • How to use the molecular weight standards to estimate the size of proteins in a sample 

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Gel Electrophoresis

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