Protein analysis techniques

Pouring an SDS-PAGE Gel

No items found.

Learn about the different roles of the separating and stacking layers within an SDS-PAGE gel and the practical steps involved in pouring both gel layers including the intermediate overlay stage.

Request a demo

See skills

arrow_downward

Learning outcomes

Explore the different roles of the separating and stacking gel layers, and overlay in between.
Set up the plates ready to pour the SDS-PAGE gel.
Pour each layer in turn, checking for leaks and including the comb.

No items found.

chevron_left

chevron_right

Skills

Concepts, Principles and Theories

Why a SDS-PAGE gel has both a stacking layer and a separating layer

Using Instruments and Equipment

How to insert the plates into the casting frame and stand and test for leaks
How to prepare the separating layer
Why an overlay is required
How to prepare the stacking layer
Why APS and TEMED must be added just before the layer is poured

Learn more about our approach to skills

Student response from Students Insights Survey

“I like that it goes through all the steps, so you have a better understanding of what you have to do for the actual lab... It makes me feel more prepared.”

Details

Degrees

Biological science

Biomedical science

Foundation science

Discipline

Molecular biology

Topic

Protein analysis techniques

Purchase option

Bioscience LabSim Library

Related products

Protein analysis techniques

SDS-PAGE Analysis

LabSim

Protein analysis techniques

SDS-PAGE Staining

LabSim

Protein analysis techniques

Loading and Running an SDS-PAGE Gel

LabSim

Protein analysis techniques

SDS-PAGE Theory: Separating Proteins by Size

LabSim

What are LabSims?

Our virtual lab simulations enhance student learning by building confidence in practical skills.

Enhance student skills and accelerate learning with multiple opportunities to practise
Boost confidence and competency with enhanced support and personalised feedback
Engage and motivate with enjoyable, challenging learning experiences

LabSim product page

What are Smart Worksheets?

Online, autograded assessment tool for advanced data analysis and calculation practice

Improve the student experience of assessment with immediate, targeted feedback
Save academic time by minimising repetitive marking for staff
Gain immediate insight into student progress with individual performance data

Smart Worksheets product page

Integrate with your LMS

Our products integrate with all major learning platforms, including Canvas, Blackboard, Moodle, Sakai and Brightspace or without any LMS using our LearnSci Direct Platform.

Learn more about LearnSci Direct.

See how our resources can enhance your student experiences

Try our resources for free
Get a live demonstration of our products
Become a partner and join our community
Talk to our team of experts

Request a demo

Image of a smiling student with a microscope