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Gel Electrophoresis

Pouring an SDS-PAGE Gel

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Learn about the different roles of the separating and stacking layers within an SDS-PAGE gel and the practical steps involved in pouring both gel layers including the intermediate overlay stage.

Learning Outcomes

  • Explore the different roles of the separating and stacking gel layers, and overlay in between
  • Set up the plates ready to pour the SDS-PAGE gel
  • Pour each layer in turn, checking for leaks and including the comb
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Concepts Principles and Theories

  • Why a SDS-PAGE gel has both a stacking layer and a separating layer 

Using Instruments and Equipment

  • How to insert the plates into the casting frame and stand and test for leaks 
  • How to prepare the separating layer 
  • Why an overlay is required 
  • How to prepare the stacking layer 
  • Why APS and TEMED must be added just before the layer is poured

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Gel Electrophoresis

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