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Gel Electrophoresis

SDS-PAGE Analysis

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Learn how to estimate the molecular weight (MW) of an unknown protein using SDS-PAGE. Measure migration distances of MW standards and calculate the Rf values. Plot the data on an MW standard curve to estimate the molecular weight of the unknown protein.

Learning Outcomes

  • Measure the migration distances of a set of molecular weight standards, and use these to calculate their relative migration distances
  • Plot a molecular weight standard curve
  • Use the standard curve to estimate the molecular weight of a protein in your sample given its relative migration distance
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Obtaining and Recording Data

  • How to measure the distance the dye front and molecular weight standards have migrated 

Manipulating and Presenting Data

  • How to calculate the Rf value for the molecular weight standards 
  • What should be plotted on the axes of a molecular weight standard curve

Interpreting and Analysing Data

  • How to use a standard curve to estimate the molecular weight of an unknown protein 
  • How to identify an unknown protein in the sample based on the molecular weight

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Gel Electrophoresis

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