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Protein analysis techniques

SDS-PAGE Analysis

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Learn how to estimate the molecular weight (MW) of an unknown protein using SDS-PAGE. Measure migration distances of MW standards and calculate the Rf values. Plot the data on an MW standard curve to estimate the molecular weight of the unknown protein.
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Learning outcomes

  • Measure the migration distances of a set of molecular weight standards, and use these to calculate their relative migration distances.
  • Plot a molecular weight standard curve.
  • Use the standard curve to estimate the molecular weight of a protein in your sample given its relative migration distance.
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Skills

Obtaining and Recording Data

  • How to measure the distance the dye front and molecular weight standards have migrated 

Manipulating and Presenting Data

  • How to calculate the Rf value for the molecular weight standards 
  • What should be plotted on the axes of a molecular weight standard curve

Interpreting and Analysing Data

  • How to use a standard curve to estimate the molecular weight of an unknown protein 
  • How to identify an unknown protein in the sample based on the molecular weight

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Details

Degrees

Biological science
Biomedical science
Foundation science

Discipline

Molecular biology

Topic

Protein analysis techniques

Purchase option

Bioscience LabSim Library

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Biological science
Biomedical science
Foundation science
sds-page
Molecular biology
Bioscience LabSim Library
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