< Back to the full list

Gel Electrophoresis

SDS-PAGE Analysis

No items found.

Learn how to estimate the molecular weight (MW) of an unknown protein using SDS-PAGE. Measure migration distances of MW standards and calculate the Rf values. Plot the data on an MW standard curve to estimate the molecular weight of the unknown protein.

Learning Outcomes

  • Measure the migration distances of a set of molecular weight standards, and use these to calculate their relative migration distances
  • Plot a molecular weight standard curve
  • Use the standard curve to estimate the molecular weight of a protein in your sample given its relative migration distance
No items found.
chevron_left
chevron_right

Skills

Obtaining and Recording Data

  • How to measure the distance the dye front and molecular weight standards have migrated 

Manipulating and Presenting Data

  • How to calculate the Rf value for the molecular weight standards 
  • What should be plotted on the axes of a molecular weight standard curve

Interpreting and Analysing Data

  • How to use a standard curve to estimate the molecular weight of an unknown protein 
  • How to identify an unknown protein in the sample based on the molecular weight

Learn more about our approach to skills
Student response from our Student Insights pilot survey

“I find using LabSims really helpful as preparation before a practical. It's useful to be able to carry out some of the practical techniques online before completing them in the lab, and they make me feel more confident about attempting these techniques during our lab sessions.”

Details

Degrees

Biochemistry
Biological Science
Biomedical Science
Forensic Science
Pharmacological Science

Modules

Biological Chemistry

Topic

Gel Electrophoresis

Purchase option

Bioscience LabSim Library

Related products

What are LabSims?

Our virtual lab simulations enhance student learning by building confidence in practical skills.
  • Enhance student skills and accelerate learning with multiple opportunities to practise
  • Boost confidence and competency with enhanced support and personalised feedback
  • Engage and motivate with enjoyable, challenging learning experiences
LabSim product page

What are Smart Worksheets?

Online, autograded assessment tool for advanced data analysis and calculation practice
  • Improve the student experience of assessment with immediate, targeted feedback
  • Save academic time by minimising repetitive marking for staff
  • Gain immediate insight into student progress with individual performance data
Smart Worksheets product page

Integrate with your LMS

Our products integrate with all major learning platforms, including Canvas, Blackboard, Moodle, Sakai and Brightspace or without any LMS using our LearnSci Direct Platform.
Learn more about LearnSci Direct.

See how our resources can enhance your student experiences

  • Try our resources for free
  • Get a live demonstration of our products
  • Become a partner and join our community
  • Talk to our team of experts
Request a demo
Image of a smiling student with a microscope