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Gel Electrophoresis

SDS-PAGE Theory: Separating Proteins by Size

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Learn about the different components of SDS-PAGE, the effects of SDS on protein charge and folding and the composition of the polyacrylamide gel. Explore how the size of a protein affects the distance travelled through the gel mesh.

Learning Outcomes

  • Connect the component parts of the acronym SDS-PAGE to key features of the protocol
  • Explore the effect of SDS on protein samples
  • Explore the nature of the polyacrylamide gel
  • Explore the role of electrophoresis
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Concepts Principles and Theories

  • What SDS-PAGE stands for 
  • How SDS affects a proteins charge and folding 
  • What is the optimal combination of SDS-PAGE gel reagents 
  • How the size of a protein affects the distance travelled through the gel mesh

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