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Molecular Cloning

Miniprep 1: Isolate Plasmid DNA

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Learn the key steps involved in isolating plasmid DNA in a miniprep protocol. Test your knowledge and understanding with interactive animations and questions.

Learning Outcomes

  • Collect bacterial cells containing plasmid
  • Break open cells to release their contents
  • Isolate plasmid DNA from other cell contents using the alkaline lysis method
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Obtaining and Recording Data

  • Which reagents and processes are required to isolate plasmid DNA 
  • When to discard the supernatant and when to discard the pellet 
  • Why it is important to thoroughly mix the contents following resuspension, lysis and neutralisation steps
  • When it is appropriate to vortex samples and when they should be gently inverted

Concepts Principles and Theories

  • What is happening on a molecular level during centrifugation, resuspension, lysis and neutralisation steps

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